Composite
Part:BBa_K322312:Design
Designed by: Maria Kowal Group: iGEM10_Edinburgh (2010-09-25)
artificial operon assembled from luxCD and luxE
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 2410
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3042
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1332
Design Notes
Notes: needed one XbaI site in luxD removed. Forward sequence is as expected. Reverse sequence does not end with TAATAA of luxE, but rather has some extra DNA, as shown, as for some reason the reverse primer was designed further downstream.
Source
Source: artificial operon assembled from luxCD and luxE of Xenorhabdus (Photorhabdus) luminescens Hb.